Western Bot. The final is decided with a single final match. A western blot experiment or western blotting also called immunoblotting because an antibody is used to specifically detect its antigen was introduced by Towbin et al.
It is an important technique used in cell. By using a western blot researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The immunoassay uses a membrane made of nitrocellulose or PVDF polyvinylidene fluoride.
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By using a western blot researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. Western blotting also called immunoblotting because an antibody is used to specifically detect its antigen was introduced by Towbin et al. Western Blotting Workflow Steps. The specificity of the antibody-antigen interaction enables a target protein to be identified in the midst of a complex protein mixture such as cell or tissue lysate.